This web page was produced as an assignment for Genetics 564, an undergraduate course at UW-Madison, Genetics 564
How can PSAP be studied?
Many genomics and proteomic techniques have been developed in order to study the activity of genes and proteins. Some of the most common techniques include whole genome sequencing, quantitative mass spectrometry, RNA sequencing, and utilizing model organisms.
Whole genome sequencing and RNA sequencing
Advances in technology have significantly reduced the cost to sequence the human genome. Illumina next generation sequencing can be used in genome wide association studies in order to identify regions of the genome that may contribute to a disease or trait of interest.
Illumina RNA sequencing can be used in order to determine which genes are being expressed and the amount of gene expression in a particular tissue sample. The amount of RNA in a sample is an indirect way to measure the amount of protein in a given sample.
Illumina RNA sequencing can be used in order to determine which genes are being expressed and the amount of gene expression in a particular tissue sample. The amount of RNA in a sample is an indirect way to measure the amount of protein in a given sample.
Mass spectrometry
Mass spectrometry is an incredibly powerful tool and can be used for a number of different purposes. Mass spectrometry starts by digesting proteins and the resulting peptide mixtures are fractionated by reversed-phase liquid chromatography. The mixture is then subjected to an electric potential, causing the ionization of the peptides. Mass to charge (m/z) ratios are measured from ions and the resulting data can be interpreted to determine the molecules in the sample. Mass spectrometry can be used to detect post-translational modifications to proteins (i.e. phosphorylation). For example, the phosphorylation of a tyrosine containing peptide causes a discrete mass increase of 80 Da to 42 Da [1].
Model organisms
Many diseases have been studied using animal models of the disease to test hypothesis. Specifically for Gaucher Disease, a transgenic mouse model has already been created which models the symptoms of the disease. In this model the mouse homolog for GBA is mutated [2] .
No information was found searching PubChem and Chembank for information about the chemical genetic of PSAP.
No information was found searching NCBI for Gene Expression Omnibus (GEO) of PSAP.
No information was found searching NCBI for Gene Expression Omnibus (GEO) of PSAP.
Sources
1. http://www.nature.com/nrg/journal/v10/n9/full/nrg2633.html
2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830832/
2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830832/
Mitchell Coplan, [email protected], 5/10/15, gen564